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Amir Khan will meet seven-time world champion Marco Antonio Barrera on 14 March at a unspecified British venue.
The 34-year-old Mexican is a former champion at three weights and has won 64 of his 71 fights.
"It's a big fight for Amir who's been champing at the bit. It's the right fight at this stage of his career," said promoter Frank Warren.
"It'll bring out the best in him. This puts him well and truly on the world stage," he added.
Squaring up to Barrera is undoubtedly the biggest test that Khan, 22, has faced so far in his career.
Last time out in December, Khan beat Irishman Oisin Fagan in his first bout since suffering the first loss of his career - a first-round knockout at the hands of Breidis Prescott last September.
The Olympic lightweight silver medallist split from trainer Jorge Rubio after that defeat and is now guided by Freddie Roach.
Barrera, who inflicted Prince Naseem Hamed's first career defeat in 2001, won his first world title in 1995, and over the years he has faced the likes of Manny Pacquiao, Juan Manuel Marquez and Erik Morales.
BBC SPORT | Boxing | Khan to face ring legend Barrera
The 34-year-old Mexican is a former champion at three weights and has won 64 of his 71 fights.
"It's a big fight for Amir who's been champing at the bit. It's the right fight at this stage of his career," said promoter Frank Warren.
"It'll bring out the best in him. This puts him well and truly on the world stage," he added.
Squaring up to Barrera is undoubtedly the biggest test that Khan, 22, has faced so far in his career.
Last time out in December, Khan beat Irishman Oisin Fagan in his first bout since suffering the first loss of his career - a first-round knockout at the hands of Breidis Prescott last September.
The Olympic lightweight silver medallist split from trainer Jorge Rubio after that defeat and is now guided by Freddie Roach.
Barrera, who inflicted Prince Naseem Hamed's first career defeat in 2001, won his first world title in 1995, and over the years he has faced the likes of Manny Pacquiao, Juan Manuel Marquez and Erik Morales.
BBC SPORT | Boxing | Khan to face ring legend Barrera